| The STENOFOLIA gene from Medicago alters leaf width, flowering time and chlorophyll content in transgenic wheat.,Plant Biotechnology Journal | 您所在的位置：网站首页 › 植物stf › The STENOFOLIA gene from Medicago alters leaf width, flowering time and chlorophyll content in transgenic wheat.,Plant Biotechnology Journal |
The STENOFOLIA gene from Medicago alters leaf width, flowering time and chlorophyll content in transgenic wheat.,Plant Biotechnology Journal
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Molecular genetic analyses revealed that the WUSCHEL-related homeobox (WOX) gene superfamily regulates several programs in plant development. Many different mechanisms are reported to underlie these alterations. The WOX family member STENOFOLIA (STF) is involved in leaf expansion in the eudicot Medicago truncutula. Here, we report that when this gene was ectopically expressed in a locally adapted hard red winter wheat cultivar (Triticum aestivum), the transgenic plants showed not only widened leaves but also accelerated flowering and increased chlorophyll content. These desirable traits were stably inherited in the progeny plants. STF binds to wheat genes that have the (GA)n /(CT)n DNA cis element, regardless of sequences flanking the DNA repeats, suggesting a mechanism for its pleiotropic effects. However, the amino acids between position 91 and 262 in the STF protein that were found to bind with the (GA)n motif have no conserved domain with any other GAGA-binding proteins in animals or plants. We also found that STF interacted with a variety of proteins in wheat in yeast 2 hybrid assays. We conclude that the eudicot STF gene binds to (GA)n /(CT)n DNA elements and can be used to regulate leaf width, flowering time and chlorophyll content in monocot wheat.
中文翻译：
分子遗传学分析显示，与WUSCHEL相关的同源盒（WOX）基因超家族调节植物发育中的多个程序。据报道，许多不同的机制是这些改变的基础。WOX家族成员STENOFOLIA（STF）参与了双子叶苜蓿截叶草的叶片扩展。在这里，我们报告说，当该基因在当地适应的硬红冬小麦品种（Triticum aestivum）中异位表达时，转基因植物不仅显示出叶宽，而且还加速了开花并增加了叶绿素含量。这些理想的性状在后代植物中稳定地遗传。STF与具有（GA）n /（CT）n DNA顺式元件的小麦基因结合，而与位于DNA重复序列侧翼的序列无关，这提示了其多效作用的机制。然而，被发现与（GA）n基序结合的STF蛋白中91和262位之间的氨基酸与动植物中的任何其他GAGA结合蛋白均不具有保守结构域。我们还发现，在酵母2杂交试验中，STF与小麦中的多种蛋白质相互作用。我们得出的结论是，双子叶植物STF基因与（GA）n /（CT）n DNA元素结合，可用于调节单子叶小麦的叶宽，开花时间和叶绿素含量。 |
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